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Hsf and Hsp gene families in Populus: genome-wide identification, organization and correlated expression during development and in stress responses
- Source :
- BMC Genomics
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Background Heat shock proteins (Hsps) are molecular chaperones that are involved in many normal cellular processes and stress responses, and heat shock factors (Hsfs) are the transcriptional activators of Hsps. Hsfs and Hsps are widely coordinated in various biological processes. Although the roles of Hsfs and Hsps in stress responses have been well characterized in Arabidopsis, their roles in perennial woody species undergoing various environmental stresses remain unclear. Results Here, a comprehensive identification and analysis of Hsf and Hsp families in poplars is presented. In Populus trichocarpa, we identified 42 paralogous pairs, 66.7% resulting from a whole genome duplication. The gene structure and motif composition are relatively conserved in each subfamily. Microarray and quantitative real-time RT-PCR analyses showed that most of the Populus Hsf and Hsp genes are differentially expressed upon exposure to various stresses. A coexpression network between Populus Hsf and Hsp genes was generated based on their expression. Coordinated relationships were validated by transient overexpression and subsequent qPCR analyses. Conclusions The comprehensive analysis indicates that different sets of PtHsps are downstream of particular PtHsfs and provides a basis for functional studies aimed at revealing the roles of these families in poplar development and stress responses. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-1398-3) contains supplementary material, which is available to authorized users.
- Subjects :
- endocrine system
Transcription, Genetic
Amino Acid Motifs
Heat shock protein (Hsp)
Biology
Genes, Plant
Bioinformatics
Expression analysis
Conserved sequence
Gene Expression Regulation, Plant
Stress, Physiological
Gene Duplication
Heat shock protein
Arabidopsis
Genetics
Coexpression
Gene family
Gene Regulatory Networks
Gene
Conserved Sequence
Phylogeny
Regulation of gene expression
Gene Expression Profiling
fungi
Chromosome Mapping
biology.organism_classification
Gene expression profiling
Populus
Organ Specificity
Multigene Family
Heat shock factor (Hsf)
DNA microarray
Genome, Plant
Research Article
Biotechnology
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....54a1597c3be3df128393df1213974422