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UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro
- Source :
- Oncology Reports. 36:2800-2806
- Publication Year :
- 2016
- Publisher :
- Spandidos Publications, 2016.
-
Abstract
- The present study was conducted to investigate the molecular mechanism of urotensin II (UII) and its receptor, G protein‑coupled receptor 14 (GPR14), in colonic inflammation. Urantide, a special antagonist of GPR14, and GPR14-siRNA were used to inhibit GPR14 signaling in dextran sulfate sodium (DSS)‑induced inflammation in mice and Caco-2 cells. The results showed that urantide alleviated rectal bleeding, histological injury and production of interleukin (IL)-17 and tumor necrosis factor‑α (TNF‑α) caused by DSS in mice. GPR14-siRNA transfection subsequent with GPR14 inhibition reduced DSS-induced interferon-γ (IFN)-γ production in Caco-2 cells. Meanwhile, both in vivo and in vitro data demonstrated that inhibition of UII/GPR14 alleviated nuclear factor-κB (NF-κB) activation caused by DSS. In conclusion, UII/GPR14 signaling was involved in the DSS-induced colonic inflammation and its inhibition may serve as a potential therapeutic target, which may be associated with the NF-κB signaling pathway.
- Subjects :
- 0301 basic medicine
Cancer Research
Colon
Urotensins
Hemorrhage
Inflammation
Biology
030226 pharmacology & pharmacy
Receptors, G-Protein-Coupled
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Interferon
medicine
Animals
Humans
Receptor
Tumor Necrosis Factor-alpha
Dextran Sulfate
Interleukin-17
NF-kappa B
Rectum
Interleukin
NF-κB
General Medicine
030104 developmental biology
Oncology
chemistry
Cancer research
Tumor necrosis factor alpha
Interleukin 17
Caco-2 Cells
Signal transduction
medicine.symptom
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....54a9e4a5397b481a0f1faae0c1c96302