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Molecular characterization of the liver-expressed antimicrobial peptide 2 (LEAP-2) in a teleost fish, Plecoglossus altivelis: Antimicrobial activity and molecular mechanism
- Source :
- Molecular Immunology. 65:406-415
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Liver-expressed antimicrobial peptide 2 (LEAP-2) is widespread in fish and plays an important role in the host's innate immune system. In this study, full-length cDNA for LEAP-2 (PaLEAP-2) gene was cloned and sequenced from ayu, Plecoglossus altivelis. PaLEAP-2 mRNA was detected in a wide range of tissues, with the highest level of transcripts found in the liver. Upon induction by Vibrio anguillarum, its expression significantly increased in the liver, kidney, spleen, gill, and heart, but decreased in the intestine. The PaLEAP-2 mature peptide was chemically synthesized; it exhibited selective antimicrobial activity against various bacteria in vitro. PaLEAP-2 at high concentration reduced the bacterial load and improved the survival rate of V. anguillarum-infected ayu. Moreover, it inhibited the expression of mRNAs for TNF-α and IL-1β in V. anguillarum-infected ayu, both at high and low concentrations. PaLEAP-2 induced hydrolysis of the pET-22b plasmid DNA and bacterium genomic DNA. These results suggest that PaLEAP-2 plays a role in ayu immune responses against bacterial infection.
- Subjects :
- Fish Proteins
Vibrio anguillarum
Interleukin-1beta
Immunology
Biology
Liver-expressed antimicrobial peptide
Microbiology
Fish Diseases
Immune system
Complementary DNA
Gene expression
Animals
RNA, Messenger
Molecular Biology
Vibrio
Innate immune system
Tumor Necrosis Factor-alpha
Fishes
biology.organism_classification
Antimicrobial
Molecular biology
Gene Expression Regulation
Liver
Organ Specificity
Vibrio Infections
Plecoglossus altivelis
Antimicrobial Cationic Peptides
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....54b83341d80573d0cd5342e035f8eb22