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Effect of macrophage colony-stimulating factor on in vitro osteoclast generation and bone resorption
- Source :
- Endocrinology. 130(1)
- Publication Year :
- 1992
-
Abstract
- To investigate the role of recombinant human macrophage colony-stimulating factor (rhM-CSF) on in vitro bone resorption, two bone explants, each at a different developmental stage, were adopted, namely 1) radii and 2) metatarsals of 17-day-old embryonic mice. At this stage of gestation, bone resorption in the radii is exclusively dependent upon fusion of osteoclast precursors and activation of mature osteoclasts, whereas in metatarsals it is dependent upon the generation of new osteoclasts. rhM-CSF showed no effect in radii, but stimulated 45Ca release in metatarsals, when they were either intact or periosteum stripped in coculture with embryonal liver as a source of hemopoietic progenitors. The rhM-CSF-induced increase in 45Ca release was paralleled by a higher number of osteoclasts. The stimulating effect was found to be in a concentration range between 250-500 U/ml M-CSF. The action of rhM-CSF was blocked by irradiation, indicating that it is dependent upon cell proliferation. These results, thus, show that M-CSF stimulates bone resorption only when it is dependent on generation of new osteoclasts. M-CSF does not appear to have any effect on the activity of mature osteoclasts. The mechanism of action might be direct on osteoclast precursors or indirect on accessory cells influencing osteoclast generation.
- Subjects :
- Macrophage colony-stimulating factor
medicine.medical_specialty
medicine.medical_treatment
Osteoclasts
Biology
Bone resorption
Mice
Endocrinology
Osteoclast
Pregnancy
Internal medicine
medicine
Animals
Bone Resorption
Cells, Cultured
Periosteum
Cell growth
Macrophage Colony-Stimulating Factor
Embryonic stem cell
Recombinant Proteins
Resorption
medicine.anatomical_structure
Cytokine
Parathyroid Hormone
Calcium
Female
Subjects
Details
- ISSN :
- 00137227
- Volume :
- 130
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....550d83d2d9008f4e143f3dc33bae8cb9