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Peripheral T-Cells, B-Cells, and Monocytes from Multiple Sclerosis Patients Supplemented with High-Dose Vitamin D Show Distinct Changes in Gene Expression Profiles

Authors :
Dohyup Kim
Emily E. Witt
Simone Schubert
Elias Sotirchos
Pavan Bhargava
Ellen M. Mowry
Karen Sachs
Biter Bilen
Lawrence Steinman
Avni Awani
Zihuai He
Peter A. Calabresi
Keith Van Haren
Source :
Nutrients; Volume 14; Issue 22; Pages: 4737
Publication Year :
2022
Publisher :
Multidisciplinary Digital Publishing Institute, 2022.

Abstract

Vitamin D is a steroid hormone that has been widely studied as a potential therapy for multiple sclerosis and other inflammatory disorders. Pre-clinical studies have implicated vitamin D in the transcription of thousands of genes, but its influence may vary by cell type. A handful of clinical studies have failed to identify an in vivo gene expression signature when using bulk analysis of all peripheral immune cells. We hypothesized that vitamin D’s gene signature would vary by immune cell type, requiring the analysis of distinct cell types. Multiple sclerosis patients (n = 18) were given high-dose vitamin D (10,400 IU/day) for six months as part of a prospective clinical trial (NCT01024777). We collected peripheral blood mononuclear cells from participants at baseline and again after six months of treatment. We used flow cytometry to isolate three immune cell types (CD4+ T-cells, CD19+ B-cells, CD14+ monocytes) for RNA microarray analysis and compared the expression profiles between baseline and six months. We identified distinct sets of differentially expressed genes and enriched pathways between baseline and six months for each cell type. Vitamin D’s in vivo gene expression profile in the immune system likely differs by cell type. Future clinical studies should consider techniques that allow for a similar cell-type resolution.

Details

Language :
English
ISSN :
20726643
Database :
OpenAIRE
Journal :
Nutrients; Volume 14; Issue 22; Pages: 4737
Accession number :
edsair.doi.dedup.....552c3a0a2dc5195ccb1068bfded4b751
Full Text :
https://doi.org/10.3390/nu14224737