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A chromatin loop represses WUSCHEL expression in Arabidopsis
- Source :
- The Plant journal : for cell and molecular biology. 94(6)
- Publication Year :
- 2017
-
Abstract
- WUSCHEL (WUS) is critical for plant meristem maintenance and determinacy in Arabidopsis, and the regulation of its spatiotemporal expression patterns is complex. We previously found that AGAMOUS (AG), a key MADS-domain transcription factor in floral organ identity and floral meristem determinacy, can directly suppress WUS expression through the recruitment of the Polycomb group (PcG) protein TERMINAL FLOWER 2 (TFL2, also known as LIKE HETEROCHROMATIN PROTEIN 1, LHP1) at the WUS locus; however, the mechanism by which WUS is repressed remains unclear. Here, using chromosome conformation capture (3C) and chromatin immunoprecipitation 3C, we found that two specific regions flanking the WUS gene body bound by AG and TFL2 form a chromatin loop that is directly promoted by AG during flower development in a manner independent of the physical distance and sequence content of the intervening region. Moreover, AG physically interacts with TFL2, and TFL2 binding to the chromatin loop is dependent on AG. Transgenic and CRISPR/Cas9-edited lines showed that the WUS chromatin loop represses gene expression by blocking the recruitment of RNA polymerase II at the locus. The findings uncover the WUS chromatin loop as another regulatory mechanism controlling WUS expression, and also shed light on the factors required for chromatin conformation change and their recruitment.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chromatin Immunoprecipitation
Chromosomal Proteins, Non-Histone
Arabidopsis
RNA polymerase II
Plant Science
Flowers
01 natural sciences
AGAMOUS Protein, Arabidopsis
Chromosome conformation capture
03 medical and health sciences
Gene Expression Regulation, Plant
CRISPR-Associated Protein 9
Genetics
Transcription factor
Homeodomain Proteins
biology
Agamous
Arabidopsis Proteins
fungi
food and beverages
Cell Biology
Meristem maintenance
Chromatin
Cell biology
030104 developmental biology
Floral meristem determinacy
biology.protein
Chromatin Loop
CRISPR-Cas Systems
Chromatin immunoprecipitation
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1365313X
- Volume :
- 94
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.doi.dedup.....553dd44c6976285f1ee59ff4fd93465d