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Crenosoma vulpis in wild and domestic carnivores from Italy: a morphological and molecular study
- Publication Year :
- 2015
-
Abstract
- Crenosoma vulpis is a metastrongyloid nematode primarily associated with respiratory tract infections of red foxes in North America and Europe. Sporadic cases have also been reported in domestic dogs. The present study aimed to provide morphological, molecular, and epidemiological data on the geographical distribution of this nematode throughout Italy. From 2012 to 2014, 12 of the 138 foxes examined, three dogs and one badger scored positive for C. vulpis. Forty adults were isolated from foxes and the badger, whereas first-stage larvae were detected in the three dogs. All specimens were morphologically identified as C. vulpis, and 28 nematodes were also molecularly characterized by sequencing mitochondrial (12S ribosomal DNA (rDNA)) and nuclear (18S rDNA) ribosomal genes. Four haplotypes were identified based on the 12S rDNA target gene, with the most representative (78.5 %) designated as haplotype I. No genetic variability was detected for the 18S rDNA gene. The molecular identification was consistent with the distinct separation of species-specific clades inferred by the phylogenetic analyses of both mitochondrial and ribosomal genes. Data herein reported indicates that C. vulpis has a wide distribution in foxes from southern Italy, and it also occurs in dogs from southern and northern regions of the country. Practitioners should consider the occurrence of this nematode in the differential diagnosis of canine respiratory disease, particularly in dogs living close to rural areas where foxes are present.
- Subjects :
- Male
12S rDNA
Veterinary medicine
Badger
Zoology
Foxes
Helminth genetics
Crenosoma vulpi
Red fox
DNA, Ribosomal
Morphological identification
Dogs
Species Specificity
biology.animal
Mustelidae
RNA, Ribosomal, 18S
Dog
Animals
Dog Diseases
Nematode Infections
Ribosomal DNA
Phylogeny
General Veterinary
Phylogenetic tree
biology
18S rDNA
Lungworm
Haplotype
General Medicine
Ribosomal RNA
DNA, Helminth
biology.organism_classification
Metastrongyloidea
Infectious Diseases
Nematode
Haplotypes
Italy
RNA, Ribosomal
Insect Science
Larva
Parasitology
Female
RNA, Helminth
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....556ba9afc9dbc8941a36ab3aa33fb556