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Genetic characterization of bovine viral diarrhea virus strains in Beijing, China and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle
- Source :
- Journal of Veterinary Science
- Publication Year :
- 2015
- Publisher :
- The Korean Society of Veterinary Science, 2015.
-
Abstract
- To acquire epidemiological data on the bovine viral diarrhea virus (BVDV) and identify cattle persistently infected (PI) with this virus, 4,327 samples from Holstein dairy cows were screened over a four-year period in Beijing, China. Eighteen BVD viruses were isolated, 12 from PI cattle. Based on genetic analysis of their 5'-untranslated region (5'-UTR), the 18 isolates were assigned to subgenotype BVDV-1m, 1a, 1d, 1q, and 1b. To investigate the innate immune responses in the peripheral-blood mononuclear cells of PI cattle, the expression of Toll-like receptors (TLRs), RIG-I-like receptors, interferon-α (IFN-α), IFN-β, myxovirus (influenza virus) resistance 1 (MX1), and interferon stimulatory gene 15 (ISG15) was assessed by qPCR. When compared with healthy cattle, the expression of TLR-7, IFN-α, and IFN-β mRNA was downregulated, but the expression of MX1 and ISG-15 mRNA was upregulated in PI cattle. Immunoblotting analysis revealed that the expression of interferon regulatory factor 3 (IRF-3) and IRF-7 was lower in PI cattle than in healthy cattle. Thus, BVDV-1m and 1a are the predominant subgenotypes in the Beijing region, and the strains are highly divergent. Our findings also suggest that the TLR-7/IRF-7 signaling pathway plays a role in evasion of host restriction by BVDV.
- Subjects :
- Untranslated region
viruses
Molecular Sequence Data
Biology
Peripheral blood mononuclear cell
Virus
Toll-like receptor
Interferon
medicine
Animals
Phylogeny
Dairy cattle
persistent infection
Innate immune system
General Veterinary
phylogenetic analysis
Diarrhea Virus 1, Bovine Viral
Interferon-alpha
Interferon-beta
Nucleocapsid Proteins
Virology
Immunity, Innate
Dairying
bovine viral diarrhea virus
Gene Expression Regulation
interferon regulatory factor
Beijing
Leukocytes, Mononuclear
RNA, Viral
Original Article
Bovine Virus Diarrhea-Mucosal Disease
Cattle
5' Untranslated Regions
medicine.drug
Interferon regulatory factors
Subjects
Details
- ISSN :
- 1976555X and 1229845X
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Science
- Accession number :
- edsair.doi.dedup.....5593c9a8a944829d67fdcd45b2c38f86