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Cryopreservation of Canine Ovaries by Vitrification
- Source :
- Journal of Reproduction and Development. 52:293-299
- Publication Year :
- 2006
- Publisher :
- Japanese Society of Animal Reproduction, 2006.
-
Abstract
- The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23 mice (100%) 4 weeks after the operation. The transplanted canine tissue was tightly adhered to the mouse ovary. Although antral follicle formation did not occur after grafting, proliferating cell nuclear antigen immunoreactivity was detectable in many of the granulosa cells in the primary follicles of the grafts. These results indicate that cryopreservation of the canine ovary by vitrification appears to have the potential to restore endocrine function and ovulation potential.
- Subjects :
- Male
DNA, Complementary
Cell Transplantation
media_common.quotation_subject
Ovary
Mice, SCID
Biology
Cryopreservation
Andrology
Mice
Cryoprotective Agents
Dogs
Ovarian Follicle
Mice, Inbred NOD
medicine
Animals
Vitrification
Ovarian tissue cryopreservation
Ovulation
media_common
Granulosa Cells
Histology
Embryo Transfer
Antral follicle
Immunohistochemistry
Transplantation
medicine.anatomical_structure
Immunology
Female
Animal Science and Zoology
Tissue Preservation
Subjects
Details
- ISSN :
- 13484400 and 09168818
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Journal of Reproduction and Development
- Accession number :
- edsair.doi.dedup.....55ac887afebb48bdde46c22911a8a15d