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Cell compatibility of a maghemite/polymer biomedical nanoplatform
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2015
- Publisher :
- Elsevier, 2015.
-
Abstract
- We are reporting the cytocompatibility and cellular fate of an iron oxide/polymer nanoplatform (IONP) in its most basic formulation, using both mesenchymal (vascular smooth muscle cells, VSMC), and epithelial (opossum kidney, OK) cells. The cytotoxicity and cell internalization of the nanoplatform has been evaluated in relation to time of exposure and concentration of different components. A series of samples with different iron oxide nanoparticle, sizes, hydrodynamic sizes and iron/polymer ratio have been examined. In all cases cytotoxicity is low, and it is mostly determined by the internalization rate, being higher in VSMC than in OK cells. The mean lethal dose has a very narrow threshold, and necrosis is the only cell death type. IONP uptake shows little incidence on oxidative stress, and inflammasome activation is only observed with the smaller IONP at high concentration. The internalization rate in VSMC is determined by the polymer concentration exclusively. In OK cells, internalization rate seems to increase with decreasing hydrodynamic size. Internalization occurs through clathrin-dependent endocytosis, as it is prevented by potassium depletion and chlorpromazine. IONP are directed and accumulated in lysosomes. Under IONP overload, lysosomal dysfunction would cause cell death using concentrations that are hardly achieved in vivo.<br />Financial support from the Spanish Ministry of Science and Innovation research grants BFU2009-12763/BFI, MAT2011-259911 and Project Consolider-Ingenio in Molecular Nanoscience CSD2007-00010 are gratefully acknowledged. Thanks are due to the EU-NoE MAGMANet for partly funding the project. L.M.A.A. acknowledges financial support from the Spanish Ministry of Science and Innovation FPI research fellowship.
- Subjects :
- Programmed cell death
Necrosis
Cell Survival
Polymers
media_common.quotation_subject
Cytotoxicity
Cell
Myocytes, Smooth Muscle
Toxicology
Endocytosis
Ferric Compounds
Polymer ratio
In vivo
medicine
Iron oxide
Animals
Internalization
Aorta
Cells, Cultured
media_common
Chemistry
Subcellular localization
General Medicine
Cell internalization
Rats
medicine.anatomical_structure
Biochemistry
Biophysics
Nanoparticles
medicine.symptom
Lysosomes
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....55f3e86b8eea49309621ffe78955a856