Biotransformation of Eugenol to Dehydroeugenol Catalyzed by Brassica juncea Peroxidase and its Cytotoxicity Activities

A crude peroxidase biocatalyst extracted from sawi hijau ( Brassica juncea ) was used to produce dimerization phenolic synthesized from eugenol. The result from MS and NMR analysis showed that the reaction between eugenol substrate and H 2 O 2 with crude extract enzyme produced a new chemical compound. The chemical structure of new compound showed coupled result in ortho-ortho position with C-C coupled derivative, dehydrodieugenol. It was obtained as brownish white crystal needles with yield 10%. This compound displayed higher cytotoxicity effect on T47D breast cancer cell compared to the eugenol monomer.