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LncRNA Rian ameliorates sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1 axis
- Source :
- Human Cell. 34:808-818
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Sevoflurane could stimulate neurotoxicity and result in postoperative cognitive dysfunction (POCD). Long non-coding RNAs (lncRNAs) have been implicated in the regulation of nervous system disease. This study was performed to investigate role and mechanism of lncRNA Rian (RNA imprinted and accumulated in nucleus) in sevoflurane anesthesia-induced cognitive dysfunction. Mice post-sevoflurane anesthesia showed cognitive impairments and neuronal damage and apoptosis. However, intracerebroventricularly injection with Adenovirus (Ad) for the over-expression of Rian ameliorated sevoflurane-induced neuronal damage and apoptosis. Cognitive impairments induced by sevoflurane were attenuated by injection with Ad-Rian. Moreover, transfection with Ad-Rian also protected isolated primary hippocampal neurons against sevoflurane-induced decrease of cell viability and increase of lactic acid dehydrogenase (LDH) and apoptosis. Mechanistically, Rian bind to miR-143-3p, and decreased expression of LIMK1 (Lim kinase 1) through negative regulation of miR-143-3p. Knockdown of LIMK1 aggravated sevoflurane-induced decrease of cell viability and increase of LDH and apoptosis in neurons, while over-expression attenuated LIMK1 silence-induced neuronal damage post-sevoflurane anesthesia. In conclusion, Rian demonstrated neuroprotective effects against sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1 axis, providing promising target for sevoflurane anesthesia-induced cognitive dysfunction.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell Survival
Gene Expression
Apoptosis
LIMK1
Pharmacology
Hippocampus
Neuroprotection
Sevoflurane
Mice
03 medical and health sciences
0302 clinical medicine
Postoperative Cognitive Complications
medicine
Animals
Viability assay
Neurons
Gene knockdown
L-Lactate Dehydrogenase
business.industry
Neurotoxicity
Lim Kinases
Nuclear Proteins
Cell Biology
medicine.disease
MicroRNAs
Neuroprotective Agents
030104 developmental biology
030220 oncology & carcinogenesis
Anesthetics, Inhalation
business
Postoperative cognitive dysfunction
medicine.drug
Subjects
Details
- ISSN :
- 17490774
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Human Cell
- Accession number :
- edsair.doi.dedup.....562a24b1610a89042b60b6565685604b