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Depth-resolved cellular microrheology using HiLo microscopy

Authors :
Peter T. C. So
Hayden Huang
Jarett Michaelson
Heejin Choi
Source :
Biomedical Optics Express
Publication Year :
2012

Abstract

It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 μm or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.

Details

ISSN :
21567085
Volume :
3
Issue :
6
Database :
OpenAIRE
Journal :
Biomedical optics express
Accession number :
edsair.doi.dedup.....56ad19ade1b0000e9d49fbafc30c21a4