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Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19
- Source :
- Diagnostic Microbiology and Infectious Disease
- Publication Year :
- 2021
- Publisher :
- Elsevier Inc., 2021.
-
Abstract
- Detection of SARS-CoV-2 RNA in nasopharyngeal samples using the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the gold standard for diagnosing COVID-19. Determination of SARS-CoV-2 RNA by rRT-PCR sometimes results in an inconclusive test result due to a high cycle threshold-value. We retrospectively analyzed 30,851 SARS-CoV-2 rRT-PCR test results. Borderline positivity was considered as the presence of ≤25 viral copies per milliliter, while no amplification was considered as a negative test result. Of all test results, 204 were answered as borderline, of which 107 were accompanied by a follow-up test within 96 hours. Of the 107 follow-up samples, 10 (9.35%) were found positive for SARS-CoV-2. COVID-19 symptoms were not predictive for testing positive in the follow-up test. The positive SARS-CoV-2 samples in the follow-up group represented 0.92% of all positive test results, highlighting the need for retesting and increased hygienic measures for borderline SARS-CoV-2 patients [NCT04636294].
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
030106 microbiology
rRT-PCR
Real-Time Polymerase Chain Reaction
Negative Test Result
Article
molecular diagnostics
03 medical and health sciences
COVID-19 Testing
0302 clinical medicine
Internal medicine
medicine
Humans
030212 general & internal medicine
Positive test
Retrospective Studies
business.industry
SARS-CoV-2
borderline
COVID-19
Liter
General Medicine
Gold standard (test)
Test (assessment)
Reverse transcription polymerase chain reaction
body regions
Early Diagnosis
Infectious Diseases
business
Follow-Up Studies
Subjects
Details
- Language :
- English
- ISSN :
- 18790070 and 07328893
- Database :
- OpenAIRE
- Journal :
- Diagnostic Microbiology and Infectious Disease
- Accession number :
- edsair.doi.dedup.....56c8f19049d91eb65cf3c8dcdda177f0