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Optical coherence tomography based microangiography provides an ability to longitudinally image arteriogenesis in vivo
- Publication Year :
- 2016
-
Abstract
- Background Arteriogenesis describes the active growth of the pre-existing collateral arterioles, which is a crucial tissue-saving process in occlusive vascular diseases. New method We propose to use optical coherence tomography (OCT)-based microangiography (OMAG) to monitor arteriogenesis following artery transection in mouse ear and focal stroke in mouse brain. Results Our longitudinal mouse ear study shows that the growth phase of arteriogenesis, indicated by changes in collateral vessel diameter and velocity, occurs between 12 and 24 h after vessel transection. Additionally, the magnitude of local inflammation is consistent with the time course of arteriogenesis, judging by the tissue thickness measurement and lymphatic vessel signals in OCT. In the mouse brain study, collateral vessel morphology, blood flow velocity and directionality are identified, and an activation of the collateral flow at the arteriolo-arteriolar anastomoses (AAA) is observed during stroke. Comparison with existing methods In comparison with histology and fluorescence imaging, OCT/OMAG is completely non-invasive and capable of producing consistent results of longitudinal changes in collateral vessel morphology and vasodynamics. Conclusion OCT/OMAG is a promising imaging tool for longitudinal study of collateral vessel remodeling in small animals. This technique can be applied in guiding the in vivo experiments of arteriogenesis stimulation to treat occlusive vascular diseases, including stroke.
- Subjects :
- medicine.medical_specialty
Time Factors
Organogenesis
01 natural sciences
Article
010309 optics
03 medical and health sciences
Mice
0302 clinical medicine
Optical coherence tomography
0103 physical sciences
Lymphatic vessel
Image Processing, Computer-Assisted
Medicine
Animals
Longitudinal Studies
Ear Diseases
Stroke
Inflammation
Mice, Hairless
medicine.diagnostic_test
business.industry
General Neuroscience
Angiography
Brain
Blood flow
Arteries
Collateral circulation
medicine.disease
Mice, Inbred C57BL
Arterioles
Disease Models, Animal
medicine.anatomical_structure
Microangiography
sense organs
Radiology
Arteriogenesis
business
030217 neurology & neurosurgery
Blood Flow Velocity
Tomography, Optical Coherence
Biomedical engineering
Artery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....56ee6e692fda0ac9baac7a434061aab7