Back to Search
Start Over
Staphylococcus epidermidis polysaccharide intercellular adhesin activates complement
- Source :
- FEMS Immunology & Medical Microbiology. 63:269-280
- Publication Year :
- 2011
- Publisher :
- Oxford University Press (OUP), 2011.
-
Abstract
- Staphylococcus epidermidis is a frequent cause of nosocomial infections. The central virulence factor of S. epidermidis is biofilm formation. Polysaccharide intercellular adhesin (PIA) constitutes the major biofilm matrix-component. PIA and biofilm have been implicated in S. epidermidis evasion of host immune defence. We examined the effects of S. epidermidis PIA on the inflammatory response with focus on complement activation. We used a human whole-blood ex vivo model of infection and compared the effects of a PIA-positive S. epidermidis strain (SE1457) and its PIA-negative isogenic mutant (M10). The independent effect of purified PIA on complement activation was investigated. In glucose-rich media, the mutant formed a proteinacious DNA-rich biofilm, whereas SE1457 formed a thick PIA-biofilm. In biofilm growth, SE1457 induced a stronger activation of the complement system compared with M10. We verified that purified PIA was independently responsible for a strong activation of the complement system. In contrast, M10 induced higher granulocyte activation by expression of CD11b and higher secretion of cytokines. We conclude that PIA has potent pro-inflammatory properties by activating the complement system. However, in a complex balance of the immune response, the decreased activation of granulocytes and cytokines by a PIA biofilm may limit host eradication of S. epidermidis.
- Subjects :
- Microbiology (medical)
Granulocyte activation
Immunology
Biology
Microbiology
Virulence factor
Immune system
Staphylococcus epidermidis
Humans
Immunology and Allergy
Complement Activation
Polysaccharides, Bacterial
Biofilm
Complement System Proteins
General Medicine
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Complement system
Blood
Human Experimentation
Infectious Diseases
Integrin alpha M
Biofilms
Macrophage-1 antigen
biology.protein
Cytokines
Gene Deletion
Granulocytes
Subjects
Details
- ISSN :
- 09288244
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- FEMS Immunology & Medical Microbiology
- Accession number :
- edsair.doi.dedup.....56f81970f006a9f29a15492917de1be4