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Innate Immune Receptor Expression in Peri-Implant Tissues of Patients With Different Susceptibility to Periodontal Diseases
- Source :
- Journal of Periodontology. 84:221-229
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Although inflammation mediates the pathogenesis of periodontal diseases, the effects of innate immune responses on implant therapies have not been evaluated. Innate immune receptors, including toll-like-receptors (TLRs) and the receptor for advanced glycated end-products (RAGE), are upregulated within inflamed gingiva and are responsible for initiation of detrimental host responses. The aim of this study is to compare the expression of TLR2, TLR4, and RAGE in gingival tissues from participants susceptible to periodontitis and participants not susceptible to periodontitis before and after implant therapy.Periodontally healthy participants received implant therapy for non-periodontal edentulism. Participants susceptible to periodontitis were diagnosed with chronic periodontitis prior to implant therapy. Gingival biopsies were collected from edentulous ridges before implant installation and from peri-implant mucosa 2 months after treatment. Histology, real-time PCR, and Western blot were used to evaluate levels of inflammatory infiltrate, TLR2, TLR4, and RAGE expression.Before implant therapy, elevated levels of RAGE were detected in gingival tissues from participants susceptible to periodontitis when compared to those from participants with healthy periodontiums, whereas no differences in the expression of TLR2 or TLR4 were detected. After implant therapy, there was an upregulation of RAGE and TLR4 levels that coincided with a downregulation of TLR2 levels in biopsies from participants susceptible to periodontitis. Levels of RAGE and TLR4 remained unchanged in biopsies from participants with healthy periodontiums, whereas TLR2 levels were significantly upregulated. Histologically, post-implant biopsies from participants susceptible to periodontitis displayed higher levels of inflammatory infiltrate.Elevated levels of inflammatory potential were found after implant therapy in participants susceptible to periodontitis.
- Subjects :
- Adult
Glycation End Products, Advanced
Male
Pathology
medicine.medical_specialty
Biopsy
Receptor expression
Blotting, Western
Receptor for Advanced Glycation End Products
Gingiva
Down-Regulation
Inflammation
Real-Time Polymerase Chain Reaction
Cohort Studies
Young Adult
Periodontal Attachment Loss
medicine
Humans
Periodontal Pocket
Gingival Recession
Receptors, Immunologic
Aged
Dental Implants
Periodontitis
Toll-like receptor
Innate immune system
business.industry
Middle Aged
medicine.disease
Chronic periodontitis
Immunity, Innate
Toll-Like Receptor 2
Up-Regulation
Toll-Like Receptor 4
TLR2
Chronic Periodontitis
Immunology
Periodontics
Female
Disease Susceptibility
Implant
medicine.symptom
Gingival Hemorrhage
business
Subjects
Details
- ISSN :
- 19433670 and 00223492
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontology
- Accession number :
- edsair.doi.dedup.....57053da962132151c07778aff700264c
- Full Text :
- https://doi.org/10.1902/jop.2012.120061