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New bacterial and archaeal lineages discovered in organic rich sediments of a large tropical Bay
- Source :
- Marine Genomics. 54:100789
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The nutrient and oxygen gradient present in marine sediments promotes high levels of microbial diversity. We applied metagenomics and biogeochemical tools to analyze microbial communities in different sediment depths (0–4 m below sea floor, mbsf) from Guanabara Bay, Brazil, a brackish tropical ecosystem with a history of massive anthropogenic impacts, and a largely unknown sediment microbial diversity. Methanogens (e.g. Methanosarcinales, Methanomicrobiales) were more abundant at 1 mbsf, while sulphate-reducing microbes (Desulfurococcales, Thermoprotales, and Sulfolobales) were more abundant at deeper layers (4 mbsf; corresponding to 3 K Radiocarbon years before present, Holocene Epoch). Taxonomic analyzes and functional gene identification associated with anaerobic methane oxidation (e.g. monomethylamine methyltransferase (mtmB), trimethylamine methyltransferase (mttB) and CO dehydrogenase/acetyl-CoA synthase delta subunit) and sulfate reduction indicated the dominance of Campylobacteria (Sulfurimonas) at deeper sediment layers. Gene sequences related to assimilation of inorganic sulfur increased with depth, while organic sulfur related sequences decrease, accompanying the clear reduction in the concentration of sulfur, organic carbon and chla torwards deeper layers. Analyzes of metagenome assembled genomes also led to the discovery of a novel order within the phylum Acidobacteriota, named Guanabacteria. This novel order had several in silico phenotyping features that differentiate it from closely related phylogenetic neighbors (e.g. Acidobacteria, Aminicenantes, and Thermoanaerobaculum), including several genes (carbon monoxide dehydrogenase, CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, heterodisulfide reductase, sulfite exporter TauE/SafE family protein, sulfurtransferase) that relevant for the S and C cycles. Furthermore, the recovered Bathyarchaeota genome SS9 illustrates the methanogenic potential in deeper sediment layer.
- Subjects :
- 0106 biological sciences
Geologic Sediments
Aquatic Science
Desulfurococcales
Mbsf
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
Sulfurimonas
Botany
Genetics
Phylogeny
030304 developmental biology
0303 health sciences
Bacteria
biology
Microbiota
biology.organism_classification
Archaea
Bays
Metagenomics
Anaerobic oxidation of methane
Methanosarcinales
Metagenome
Methanomicrobiales
Brazil
Acidobacteria
Subjects
Details
- ISSN :
- 18747787
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Marine Genomics
- Accession number :
- edsair.doi.dedup.....572585e4567b671225fa020615a21af3