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Sunfish cationic amphiphiles: toward an adaptative lipoplex morphology
Sunfish cationic amphiphiles: toward an adaptative lipoplex morphology
- Source :
- Journal of the American Chemical Society, 127(29), 10420-10429. AMER CHEMICAL SOC
- Publication Year :
- 2005
-
Abstract
- A detailed physicochemical study is presented on a new class of cationic amphiphiles, Sunfish amphiphiles, recently designed, synthesized, and tested for gene delivery. These materials have two hydrophobic tails, connected to the cationic pyridinium headgroup at the 1- and 4-positions. Two extreme morphologies can be visualized, i.e. one by back-folding involving association of both tails at one side of the pyridinium ring and one by independent unfolding of the tails, the two molecular geometries leading to considerable differences in the aggregate morphology. The behavior of six members of the Sunfish family in mixtures with DOPE, applying different conditions relevant for transfection, has been studied by a combination of techniques (DLS, DSC, NMR, SAXS, Cryo-TEM, fluorescence, etc.). The effects of structural parameters such as the presence of unsaturation in the tails and length of the alkyl chains on the properties of the aggregates have been assessed. A correlation of these structural data with cellular transfection efficiencies reveals that the highest transfection efficiency is obtained with those amphiphiles that are easily hydrated, form fluid aggregates, and undergo a transition to the inverted hexagonal phase in the presence of plasmid DNA (p-DNA) at physiological ionic strength.
- Subjects :
- EFFICIENCY
Stereochemistry
LIPID-DNA COMPLEXES
MEMBRANE-FUSION
Transfection
Biochemistry
Catalysis
PHASE-BEHAVIOR
GENE DELIVERY
chemistry.chemical_compound
Colloid and Surface Chemistry
PLASMID DNA
Cations
Amphiphile
Chlorocebus aethiops
Animals
SURFACTANTS
Nuclear Magnetic Resonance, Biomolecular
Alkyl
chemistry.chemical_classification
Degree of unsaturation
Small-angle X-ray scattering
Phosphatidylethanolamines
Hexagonal phase
Cationic polymerization
Water
General Chemistry
DNA
POLYMORPHISM
chemistry
Ionic strength
COS Cells
Liposomes
Picolines
Biophysics
TRANSFECTION ACTIVITY
Pyridinium
PYRIDINIUM AMPHIPHILES
Subjects
Details
- ISSN :
- 00027863
- Volume :
- 127
- Issue :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....573deae16b2e73bc993e26bde544a629