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Gonadotropin releasing hormone enhances polyphosphoinositide hydrolysis in rat pituitary cells
- Source :
- Biochemical and biophysical research communications. 134(2)
- Publication Year :
- 1986
-
Abstract
- Addition of gonadotropin releasing hormone to myo-[2-3H]inositol-prelabeled rat pituitary cells in primary culture evoked a dose-dependent increase of the accumulation of [3H]inositol phosphates with a rise of inositol triphosphate within 30 sec of stimulation, followed by a rise in inositol diphosphate and inositol monophosphate. Inositol phosphate accumulation was enhanced up to 5-to-8-fold and was time-dependent between up to 15 min incubation without further increase beyond this time period. Without preincubation with LiCl2, there was no measurable increase of GnRH-induced inositol phosphate accumulation compared to controls. The presence of calcium in the incubation medium did not affect the increase of inositol phosphates. These data give evidence, that polyphosphoinositide breakdown may be an early step in the action of gonadotropin releasing hormone on gonadotropin secretion.
- Subjects :
- medicine.medical_specialty
Pituitary gland
Inositol Phosphates
Biophysics
chemistry.chemical_element
Stimulation
Gonadotropin-releasing hormone
Biology
Calcium
Lithium
Phosphatidylinositols
Biochemistry
Gonadotropin-Releasing Hormone
chemistry.chemical_compound
Chlorides
Thyrotropin-releasing hormone receptor
Pituitary Gland, Anterior
Internal medicine
medicine
Animals
Inositol
Inositol phosphate
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
Hydrolysis
Cell Biology
Phosphoric Monoester Hydrolases
Gonadotropin secretion
Rats
medicine.anatomical_structure
Endocrinology
chemistry
Female
Sugar Phosphates
Lithium Chloride
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 134
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....5757ad253034d9bd17f7ca0dca0cdc51