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Weight gain and inflammation regulate aromatase expression in male adipose tissue, as evidenced by reporter gene activity
- Source :
- Europe PubMed Central
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Obesity and white adipose tissue (WAT) inflammation are associated with enhanced aromatization in women, but little is known about the regulation of aromatase (CYP19A1) gene expression in male WAT. We investigated the impact of weight gain and WAT inflammation on the regulation of CYP19A1 in males, by utilizing the hARO-Luc aromatase reporter mouse model containing a >100-kb 5′-region of the human CYP19A1 gene. We show that hARO-Luc reporter activity is enhanced in WAT of mice with increased adiposity and inflammation. Dexamethasone and TNFα, as well as forskolin and phorbol 12-myristate 13-acetate, upregulate hARO-Luc activity, suggesting the involvement of promoters I.4 and I.3/II. Furthermore, we show that diet enriched with antioxidative plant polyphenols attenuates WAT inflammation and hARO-Luc activity in obese males. In conclusion, our data suggest that obesity-associated WAT inflammation leads to increased peripheral CYP19A1 expression in males, and that polyphenol-enriched diet may have the potential to attenuate excessive aromatization in WAT of obese men.
- Subjects :
- Male
Transcriptional Activation
endocrine system
medicine.medical_specialty
Adipose Tissue, White
Gene Expression
Adipose tissue
Inflammation
White adipose tissue
ta3111
Diet, High-Fat
Weight Gain
Biochemistry
Antioxidants
Mice
chemistry.chemical_compound
Aromatase
Endocrinology
Genes, Reporter
Internal medicine
Gene expression
medicine
Animals
Obesity
Luciferases
Molecular Biology
Cells, Cultured
Reporter gene
biology
Plant Extracts
ta1182
Polyphenols
nutritional and metabolic diseases
food and beverages
chemistry
Enzyme Induction
biology.protein
Phorbol
Cytokines
Tumor necrosis factor alpha
medicine.symptom
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 412
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Endocrinology
- Accession number :
- edsair.doi.dedup.....5776ae3fe0e8cf199bc8b4bfb07c5ba7