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A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector
- Source :
- Chinese Journal of Integrative Medicine. 18:690-698
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Anthraquinones in water extract of rhubarb (WER), in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
- Subjects :
- Male
Quality Control
Emodin
Antioxidant
Traumatic brain injury
medicine.medical_treatment
Anthraquinones
Anthraquinone
Absorption
Rats, Sprague-Dawley
chemistry.chemical_compound
Cerebrospinal fluid
Limit of Detection
Photodiode array detector
medicine
Animals
Humans
Pharmacology (medical)
Rheum
Biological Products
Chromatography
Plant Extracts
Reproducibility of Results
Water
General Medicine
Reference Standards
medicine.disease
Rats
Complementary and alternative medicine
chemistry
Brain Injuries
Linear Models
Dismutase
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19930402 and 16720415
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Integrative Medicine
- Accession number :
- edsair.doi.dedup.....579790abbc5d80d3ee61db581465cc52