Back to Search
Start Over
DNA methylation state of the galectin-3 gene represents a potential new marker of thyroid malignancy
- Source :
- Oncology Letters
- Publication Year :
- 2013
-
Abstract
- In order to supplement the cytopathological assessment of thyroid tumors, there is a need for new markers to correctly diagnose malignant thyroid lesions and avoid unnecessary and potentially harmful therapies for patients. The immunohistochemical expression of galectin-3 is currently considered to be the most accurate stand-alone marker for thyroid cancer diagnosis. The aim of this study was to establish whether the methylation state of the galectin-3 gene is a candidate molecular marker for thyroid malignancy. Thyroid specimens from 50 patients were analyzed, including 5 normal thyroid, 3 goiters, 39 papillary and 3 anaplastic thyroid carcinoma cases. High-resolution methylation analyses was performed to investigate the methylation state of a large genomic region (from −89 to +408) encompassing the galectin-3 transcriptional start site. Within this region, 5 CpG sites (nucleotide positions +134, +137, +142, +147 and +156) were observed to be differentially methylated among the samples and were further analyzed by the quantitative pyrosequencing technique. The hypomethylation of the +134, +137, +142, +147 and +156 CpG sites was observed to be markedly associated with cancer. Although the methylation degree of each single site was highly variable in non-neoplastic tissues, the average methylation state of the 5 CpG sites clearly distinguished cancer from the nonneoplastic thyroid tissues.
- Subjects :
- "epigenetics"
endocrine system
Cancer Research
Pathology
medicine.medical_specialty
DNA methylation
endocrine system diseases
Thyroid
human thyroid cancer
Cancer
Articles
Methylation
Biology
medicine.disease
Molecular medicine
medicine.anatomical_structure
Oncology
CpG site
galectin-3
tumor marker
medicine
thyroid cancer
Thyroid cancer
Tumor marker
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....579ad6235368d544b2f09921bb649cb5