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Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
- Source :
- JOURNAL OF CLINICAL VIROLOGY, r-FISABIO. Repositorio Institucional de Producción Científica, instname, Journal of Clinical Virology, 134. ELSEVIER, Journal of Clinical Virology, 134:104691. ELSEVIER SCIENCE BV, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA, Digital.CSIC. Repositorio Institucional del CSIC, r-FISABIO: Repositorio Institucional de Producción Científica, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), Journal of Clinical Virology, 134:104691. Elsevier
- Publication Year :
- 2021
-
Abstract
- Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
- Subjects :
- 0301 basic medicine
Pathogen detection
Standardization
Computer science
030106 microbiology
Recommendations
INFLUENZA-A VIRUS
DIAGNOSIS
VALIDATION
DNA sequencing
03 medical and health sciences
0302 clinical medicine
SDG 3 - Good Health and Well-being
Virology
Wet lab
Viral metagenomics
030212 general & internal medicine
11832 Microbiology and virology
Laboratory methods
High-throughput sequencing
Quality assessment
Network on
High-Throughput Nucleotide Sequencing
DNA
EFFICIENT TRANSLATION
Data science
3. Good health
Infectious Diseases
Metagenomics
Viruses
Next-generation sequencing
3111 Biomedicine
DEPLETION
MESSENGER-RNA
Clinical virology
PATHOGEN DETECTION
Subjects
Details
- Language :
- English
- ISSN :
- 13866532 and 18735967
- Volume :
- 134
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Virology
- Accession number :
- edsair.doi.dedup.....57a8e5461f07041be03faaba90550bc9