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Dose-Dependent Effect of Polystyrene Microplastics on the Testicular Tissues of the Male Sprague Dawley Rats

Authors :
Arfa Tahir
Hussain Gadelkarim Ahmed
Abdul Samad
Sabahat Shahzadi
Haseeb Anwar
Humaira Rehman
Nazia Ehsan
Muhammad Umar Ijaz
Source :
Dose-Response, Dose-Response, Vol 19 (2021)
Publication Year :
2021
Publisher :
SAGE Publications, 2021.

Abstract

Due to the continuous increase in polystyrene microplastics (PS MPs) incorporation in the environment, growing number of adverse effects on living organisms and ecosystem have become a global concern. Therefore, current study was planned to elucidate the impacts of 5 different concentrations control, 2, 20, 200, and 2000 μgL-1 of PS MPs on testicular tissues of rats. PS MPs significantly reduced the activities of antioxidant enzymes (catalase, superoxide dismutase and peroxidase) as well as total protein contents, while elevated the level of lipid peroxidation and reactive oxygen species. Moreover, expressions of steroidogenic enzymes (3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein) as well as the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) in plasma, intra-testicular testosterone and plasma testosterone were reduced and a significant ( P < 0.05) reduction was noticed in the sperm count, motility and viability. Furthermore, PS MPs significantly up-regulated the expressions of Bax and caspase-3, while down-regulated the Bcl-2 expression. The histomorphological assessment revealed significant damages in the testicles as well as decrease in the number of germ cells (spermatogenic, spermatocytes and spermatids). Collectively, PS MPs generated oxidative stress (OS) and caused potential damage to the testicles of rats in a dose-dependent manner.

Details

ISSN :
15593258
Volume :
19
Database :
OpenAIRE
Journal :
Dose-Response
Accession number :
edsair.doi.dedup.....57b7e26bf4a48a2d795a0d0575e2bb12
Full Text :
https://doi.org/10.1177/15593258211019882