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Aurora B expression in normal testis and seminomas
- Source :
- The Journal of endocrinology. 181(2)
- Publication Year :
- 2004
-
Abstract
- Aurora/Ipl1-related kinases are a conserved family of proteins that have multiple functions during mitotic progression. High levels of Aurora kinases are characteristic of rapidly dividing cells and tumours. Aurora B encodes a protein that associates with condensing chromatin, concentrates at centromeres, and then relocates onto the central spindle at anaphase. In this study the expression and the localisation of Aurora B throughout germinal epithelial progression in normal testis and its neoplastic counterpart were analysed. Immunocytochemistry and RT-PCR analysis of mouse germinal epithelium cells showed the presence of Aurora B in spermatogonia and occasionally in spermatocytes. Western blot analysis revealed the typical Aurora B isoform ( approximately 41 kDa) in the same cellular types. A similar distribution was observed in human testis by immunohistochemistry. Moreover, the distribution and the expression of Aurora B were investigated in neoplasms derived from germ cells. Surgical samples of seminomas were analysed, and a high percentage of Aurora B positive cells (51%) was detected; the expression of Aurora B was significantly related to the MIB-1 proliferation marker (R=0.816). The data presented here demonstrate that Aurora B expression occurs in spermatogonial division. Furthermore, our results indicate that the expression of Aurora B is a consistent feature of human seminomas.
- Subjects :
- Germinal epithelium
Male
medicine.medical_specialty
Pathology
Cell division
spermatogenesi
Endocrinology, Diabetes and Metabolism
Immunocytochemistry
Aurora B kinase
Mice, Inbred Strains
macromolecular substances
Biology
Protein Serine-Threonine Kinases
Map kinase
Mice
Endocrinology
Testicular Neoplasms
Aurora Kinases
Spermatocytes
Internal medicine
Testis
medicine
Animals
Aurora Kinase B
Proliferation Marker
RNA, Messenger
Aurora B
Kinase
Reverse Transcriptase Polymerase Chain Reaction
Molecular biology
Immunohistochemistry
Spermatozoa
Spermatogonia
Chromatin
Seminoma
Isoenzymes
enzymes and coenzymes (carbohydrates)
Ki-67 Antigen
embryonic structures
biological phenomena, cell phenomena, and immunity
Biomarkers
Cell Division
Subjects
Details
- ISSN :
- 00220795
- Volume :
- 181
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of endocrinology
- Accession number :
- edsair.doi.dedup.....57befea4b9736c45fc8e0d933a4438b0