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Cytogenetic biomonitoring of primary school children exposed to air pollutants: micronuclei analysis of buccal epithelial cells
- Source :
- Environmental Science and Pollution Research. 21:1197-1207
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- WOS: 000329243700038<br />PubMed ID: 23884878<br />There is an increasing attempt in the world to determine the exposures of children to environmental chemicals. To analyze the genotoxic effect of air pollution, micronucleus (MN) assay was carried out in buccal epithelial cells (BECs) of children living in an urban city of Turkey. Children from two schools at urban-traffic and suburban sites were investigated in summer and winter seasons for the determination of BEC-MN frequency (per mille) and frequency of BEC with MN (per mille). The same children were also recruited for lung function measurements within a MATRA project ("Together Towards Clean Air in Eskisehir and Iskenderun") Measured NO2 and SO2 concentrations did not exceed the European Union (EU) limit levels either in urban-traffic or suburban regions. Higher O-3 concentrations were measured in the suburban site especially in the summer period. Particulate matter (PM2.5 and PM10) levels which did not differ statistically between two regions were above the EU limits in general. Although BEC-MN frequencies of children living in the suburban sites were higher in general, the difference between two regions was not significant either in the summer or winter periods. BEC-MN frequencies of the urban-traffic children were found to be significantly higher in summer period (mean +/- SD, 2.68 +/- 1.99) when compared to winter period (1.64 +/- 1.59; p = 0.004). On the other hand, no seasonality was observed for the suburban children. Similar results have been obtained in the BEC frequency with MN in our study. In summer, BEC-MN frequencies were significantly increased with the decrease in pulmonary function levels based on forced expiratory flow between 25 and 75 % of vital capacity (FEF25-75 %) levels (p < 0.05). As a conclusion, children living in urban-traffic and suburban areas in the city of EskiAYehir exhibited similar genotoxicity. Seasonal variation in genotoxicity may be interpreted as relatively high ozone levels and increasing time spent at outdoors in the summer.<br />Dutch Ministry of Foreign Affairs<br />We would like to thank Kees Meliefste and Institute for Risk Assessment Sciences for their support during field studies. We also thank very much to the school directors and teachers for their very valuable help during the sampling studies. The authors are grateful to the children and their parents for their participation in this study. We would also like to thank Mr. Salih Ergocen for performing the statistical analysis.; This study was part of the MATRA project "Together Towards Clean Air in Eskisehir and Iskenderun" financed by the Dutch Ministry of Foreign Affairs.
- Subjects :
- Male
Turkey
Micronucleus Assay
Climate
Health, Toxicology and Mutagenesis
Air pollution
medicine.disease_cause
Lung-Function
Peripheral Lymphocytes
Environmental protection
Biomonitoring
Child
Children
health care economics and organizations
media_common
Air Pollutants
Micronucleus Tests
Schools
General Medicine
Environmental-Pollutants
Dna-Damage
Pollution
Micronucleus test
Pollution Exposure
Female
Seasons
Environmental Monitoring
Buccal epithelial cells
Buccal Epithelial Cells
Adolescent
Cancer Incidence
Biology
Cytogenetics
Ozone
Animal science
Air Pollution
medicine
Humans
Environmental Chemistry
media_common.cataloged_instance
Cities
European union
Genetic-Damage
Mouth Mucosa
Micronucleus assay
Epithelial Cells
Environmental Exposure
Seasonality
Personal biomonitoring
Health-Risk
medicine.disease
Personal Biomonitoring
Polycyclic Aromatic-Hydrocarbons
Personal Exposure
Particulate Matter
Per mille
Micronucleus
Genotoxicity
Subjects
Details
- ISSN :
- 16147499, 09441344, and 23884878
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Environmental Science and Pollution Research
- Accession number :
- edsair.doi.dedup.....57d69622f1c29207448d2dc9089c3be7