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Sputum processing for evaluation of inflammatory mediators
- Source :
- Pediatric Pulmonology. 32:152-158
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- Neutrophil-dominated inflammation is prominent in the cystic fibrosis (CF) and chronic bronchitis (CB) airways. We assessed the degree of airway inflammation by measuring the sputum concentrations of interleukin (IL)-8, myeloperoxidase (MPO), and deoxyribonucleic acid (DNA). We determined the relationship among the concentrations of these mediators and investigated methodological problems that may be responsible for reported variability in measurements. Sputa obtained from 31 patients were solubilized with phosphate-buffered saline, dithiothreitol (DTT) (0.1% or 1%), or dornase alfa (0.2 mg/mL). The sputum concentration of IL-8 and MPO was measured by enzyme-linked immunosorbent assay (ELISA), and DNA was measured using microfluorimetry. There was a significant relationship among sputum IL-8, MPO, and DNA. For MPO (means +/- SD), CF was 1,392 +/- 771 vs. CB at 75 +/- 65 mcg/mL; P < 0.0001. For IL-8: CF was 239 +/- 154 vs. CB at 121 +/- 108 ng/mL; P = 0.0002. For DNA, CF was 1.707 +/- 1.25 vs. CB at 0.184 +/- 0.272 mg/mL; P < 0.0001. The MPO concentration in CF sputum was approximately double after in vitro treatment with dornase alfa (P < 0.0001). There is a greater concentration of IL-8, MPO, and DNA in CF than in CB sputa. There is a significant relationship among these inflammatory markers in sputum. DNA polymers bind myeloperoxidase in the sputum, and we speculate that treatment with dornase alfa may remove a source of MPO inhibition.
- Subjects :
- Adult
Male
Pulmonary and Respiratory Medicine
Chronic bronchitis
Adolescent
Cystic Fibrosis
Polymers
Cystic fibrosis
Dithiothreitol
Specimen Handling
chemistry.chemical_compound
medicine
Deoxyribonuclease I
Humans
Interleukin 8
Bronchitis
Child
Expectorants
Peroxidase
Inflammation
biology
business.industry
Interleukin-8
Sputum
Interleukin
Dornase alfa
DNA
medicine.disease
Molecular biology
Recombinant Proteins
chemistry
Myeloperoxidase
Pediatrics, Perinatology and Child Health
Immunology
biology.protein
Female
medicine.symptom
business
Biomarkers
medicine.drug
Subjects
Details
- ISSN :
- 10990496 and 87556863
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Pediatric Pulmonology
- Accession number :
- edsair.doi.dedup.....57e2ecdfcae147eaad17e7115a0a3157