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Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2006
- Publisher :
- Rockefeller University Press, 2006.
-
Abstract
- Tyrosine and glycine constitute 40% of complementarity determining region 3 of the immunoglobulin heavy chain (CDR-H3), the center of the classic antigen-binding site. To assess the role of DH RF1-encoded tyrosine and glycine in regulating CDR-H3 content and potentially influencing B cell function, we created mice limited to a single DH encoding asparagine, histidine, and arginines in RF1. Tyrosine and glycine content in CDR-H3 was halved. Bone marrow and spleen mature B cell and peritoneal cavity B-1 cell numbers were also halved, whereas marginal zone B cell numbers increased. Serum immunoglobulin G subclass levels and antibody titers to T-dependent and T-independent antigens all declined. Thus, violation of the conserved preference for tyrosine and glycine in DH RF1 alters CDR-H3 content and impairs B cell development and antibody production.
- Subjects :
- Molecular Sequence Data
Immunology
Article
Immunoglobulin G
Mice
Marginal zone B-cell
medicine
Animals
Immunology and Allergy
Amino Acid Sequence
Amino Acids
Tyrosine
B cell
B-Lymphocytes
Mice, Inbred BALB C
biology
Articles
Molecular biology
medicine.anatomical_structure
Biochemistry
Immunoglobulin M
Antibody Formation
Glycine
biology.protein
Immunoglobulin heavy chain
Antibody
Immunoglobulin Heavy Chains
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....57fb22635a8063c3d892ede27755252d
- Full Text :
- https://doi.org/10.1084/jem.20052217