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Surface IgM λ light chain is involved in the binding and infection of infectious bursal disease virus (IBDV) to DT40 cells
- Source :
- Virus genes. 54(2)
- Publication Year :
- 2017
-
Abstract
- Infectious bursal disease virus (IBDV) is an important immunosuppressive virus in chickens. Surface immunoglobulin M (sIgM)-bearing B lymphocytes act as the major targets of IBDV in the bursa of Fabricius, and sIgM may function as one of the membrane binding sites responsible for IBDV infection. Recently, using the virus overlay protein binding assay, the chicken λ light chain of sIgM was identified to specifically interact with IBDV in a virulence-independent manner in vitro. To further investigate sIgM λ light chain-mediated IBDV binding and infection in pre-B cells, the cell line DT40, which is susceptible to both pathogenic and attenuated IBDV, was used. Based on the RNA interference strategy, the DT40 cell line whose λ light chain of sIgM was stably knocked down, herein termed DT40LKD, was generated by the genomic integration of a specific small hairpin RNA and a green fluorescence protein co-expression construct. Flow cytometry analysis indicated that the binding of IBDV to DT40LKD cells was significantly reduced due to the loss of sIgM λ light chain. In particular, reduced viral replication was observed in IBDV-incubated DT40LKD cells, and no viral release into cell culture medium was detected by the IBDV rapid diagnostic strips. In addition, the rescue of sIgM λ light chain expression restored viral binding and replication in DT40LKD cells. These results show that sIgM λ light chain appears to be beneficial for IBDV attachment and infection, suggesting that sIgM acts as a binding site involved in IBDV infection.
- Subjects :
- 0301 basic medicine
animal structures
030106 microbiology
Virus Attachment
Immunoglobulin light chain
Virus Replication
Infectious bursal disease virus
Models, Biological
Virus
Infectious bursal disease
Cell Line
Small hairpin RNA
03 medical and health sciences
Virology
Genetics
medicine
Animals
Bursa of Fabricius
Molecular Biology
Poultry Diseases
B-Lymphocytes
biology
Genetic Complementation Test
Membrane Proteins
General Medicine
medicine.disease
Birnaviridae Infections
030104 developmental biology
Viral replication
Immunoglobulin M
Cell culture
Gene Knockdown Techniques
biology.protein
Receptors, Virus
Immunoglobulin Light Chains
RNA Interference
Antibody
Chickens
Subjects
Details
- ISSN :
- 1572994X
- Volume :
- 54
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virus genes
- Accession number :
- edsair.doi.dedup.....583b8ca0238416fcb24c67c11dd23458