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Isolation and Characterization of a Fucoidan-Degrading Marine Bacterial Strain and Its Fucoidanase
- Source :
- Marine Biotechnology. 6:335-346
- Publication Year :
- 2004
- Publisher :
- Springer Science and Business Media LLC, 2004.
-
Abstract
- A marine bacterial strain that degraded fucoidan from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) was isolated in our laboratory. The strain was gram-negative, ubiquinone 8 was the predominant respiratory quinone, and the GC-content of its genomic DNA was 36%. The cells of the strain were rod-shaped (2.0 microm long x 1.0 microm wide), and each cell was motile by means of one polar flagellum. Phylogenetic analysis of its 16S ribosomal DNA sequence indicated that it was a member of the family Alteromonadaceae. It produced a type of extracellular fucoidanase, an endosulfated fucan-digesting enzyme. The enzyme was purified with 3500-fold purity at 12.0% yield. Optimum conditions for the enzyme reaction were approximately pH 6.5 to 8.0 and temperature 30 degrees to 35 degrees C. The enzyme was activated by calcium ions, and maximum activity was observed in the presence of greater than 30 mM calcium ion.
- Subjects :
- Molecular Sequence Data
chemistry.chemical_element
Biology
Calcium
Alteromonadaceae
Phaeophyta
Applied Microbiology and Biotechnology
chemistry.chemical_compound
Polysaccharides
RNA, Ribosomal, 16S
Extracellular
Cluster Analysis
Laminariaceae
Phylogeny
DNA Primers
chemistry.chemical_classification
Base Composition
Base Sequence
Strain (chemistry)
Fucoidan
Temperature
Sequence Analysis, DNA
Hydrogen-Ion Concentration
Ribosomal RNA
biology.organism_classification
Molecular biology
Enzymes
Enzyme
chemistry
Biochemistry
Subjects
Details
- ISSN :
- 14362236 and 14362228
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Marine Biotechnology
- Accession number :
- edsair.doi.dedup.....583c88f0cfc6ee4f03576f09c1beb9f7