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Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene
- Source :
- PLANT CELL REPORTS. 21(10):988-992
- Publication Year :
- 2003
-
Abstract
- Microsomal omega-3 fatty acid desaturase is an essential enzyme in the production of the n-3 polyunsaturated fatty acid alpha-linolenic acid during the seed developing stage. We have constructed a chimeric gene consisting of a maize Ubi1-P-int and a soybean GmFAD3 cDNA, which was introduced into rice plants by Agrobacterium-mediated transformation. Ten transformants containing the chimeric gene were established and expression subsequently confirmed by Northern blotting. Furthermore, alpha-linolenic acid content of the T(1) seeds increased dramatically up to tenfold that of the control, and this phenotype was also stably inherited in the T(2) and T(3) progenies. These results demonstrate that the alpha-linolenic acid content of rice seed oil can easily be altered using the combination of a high-activity promoter and a GmFAD3 gene.
- Subjects :
- Fatty Acid Desaturases
Gene Expression
Plant Science
Chimeric gene
Complementary DNA
Plant Oils
Northern blot
RNA, Messenger
Gene
chemistry.chemical_classification
Oryza sativa
biology
Fatty Acids
food and beverages
Oryza
General Medicine
Blotting, Northern
Plants, Genetically Modified
Transformation (genetics)
Blotting, Southern
Fatty acid desaturase
chemistry
Biochemistry
RNA, Plant
Seeds
biology.protein
Soybeans
Agronomy and Crop Science
Polyunsaturated fatty acid
Subjects
Details
- Language :
- English
- Volume :
- 21
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLANT CELL REPORTS
- Accession number :
- edsair.doi.dedup.....584848ddc2ee3c5b87a3d2ab11fa644a