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Molecular cloning of haemoglobin-binding protein HgbA in the outer membrane of Actinobacillus pleuropneumoniae

Authors :
James W. Coulton
Ramakrishnan Srikumar
Leonie G. Mikael
Ali Khamessan
Peter D. Pawelek
Mario Jacques
Bernard F. Gibbs
Source :
Microbiology (Reading, England). 150(Pt 6)
Publication Year :
2004

Abstract

From the porcine pathogenActinobacillus pleuropneumoniaecultivated in iron-deficient or haem-deficient media, haemoglobin (Hb)-agarose affinity purification was exploited to isolate an outer-membrane protein of ∼105 kDa, designated HgbA. Internal peptide sequences of purified HgbA were used to design oligonucleotide primers for PCR amplification, yielding amplicons that showed partial sequences with homology tohgbAofPasteurella multocida. Upon screening two genomic libraries ofA. pleuropneumoniaeserotype 1 strain 4074, positive clones were assembled into an ORF of 2838 bp. HgbA (946 aa) includes a signal peptide of 23 aa and the deduced HgbA sequence (104 890 Da) also demonstrated a possible Ton box. The promoter region ofhgbAfromA. pleuropneumoniaeserotype 1 showed consensus for −35 and −10 sequences and a putative Fur-binding site. RT-PCR confirmed thathgbAofA. pleuropneumoniaeis upregulated in response to diminished levels of iron in the culture medium. While an internally deletedhgbAmutant was unable to use pig Hb as sole source of iron for growth, flow cytometry confirmed its Hb binding; the internally deleted sequences may not be required for Hb binding, but appear necessary for the iron supply from Hb. HgbA is required for growth ofA. pleuropneumoniaein the presence of Hb as sole iron source.

Details

ISSN :
13500872
Volume :
150
Issue :
Pt 6
Database :
OpenAIRE
Journal :
Microbiology (Reading, England)
Accession number :
edsair.doi.dedup.....5891f38152f21c722850e3fe08db527b