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Cat's whiskers (Orthosiphon stamineus) tea modulates arthritis pathogenesis via the angiogenesis and inflammatory cascade
- Source :
- BMC Complementary and Alternative Medicine
- Publication Year :
- 2016
-
Abstract
- Background Orthosiphon stamineus is used traditionally to treat gout, arthritis, and inflammatory related conditions. The in vitro anti-inflammatory effects of the plant have been scientifically investigated. The goal of the present study was to evaluate the potential of the 50% ethanol extract of O. stamineus (EOS) to treat rheumatoid arthritis. Methods Anti-arthritic activity was assessed using the in vitro heat denaturation test and the (FCA)-induced arthritis model. Efficacy was assessed by measurements of paw edema and granulation, X-ray radiography, fluorescence molecular tomography (FMT), and histological evaluation. Levels of (TNF-α), interleukin-1 (IL-1), and (COX-1 and COX-2) were analyzed in vitro in lipopolysaccharide (LPS)-stimulated human macrophage (U937). TNF-α and IL-1 levels in the serum samples of arthritic rats were also measured using an ELISA kit. Results Treatment with EOS resulted in dose-dependent inhibition of paw edema in acute and chronic models of inflammation. It also inhibited significantly the production of TNF-α, IL-1 COX-1, and COX-2 in the LPS-stimulated U937 macrophages. EOS significantly suppressed FCA-induced paw edema as well as the serum levels of TNF-α and IL-1. X-rays of the synovial joint of the hind leg showed considerable improvement in joint integrity and recovery of tibia-talus bones from degeneration and osteoporotic lesions. Histology of proximal interphalangeal joints of EOS-treated animals showed obvious protection of cartilage and soft tissue. Finally, FMT analysis strongly supported the anti-arthritic effect of EOS. EOS had high phenolic and total flavonoid content as well as strong antioxidant activity. Conclusions Results illustrated that the anti-arthritic properties of O. stamineus could be beneficial for prevention and management of rheumatoid arthritis and other chronic inflammatory disorders. Graphical abstract Illustration of the Anti- arthritis efficacy of Orthosiphon Stamineus standardized extract.
- Subjects :
- 0301 basic medicine
Male
Lipopolysaccharide
medicine.drug_class
Orthosiphon stamineus
Pro-inflammatory mediators
Arthritis
Inflammation
Pharmacology
01 natural sciences
Anti-inflammatory
Antioxidants
Arthritis, Rheumatoid
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Phenols
medicine
Animals
Humans
Orthosiphon
Anti-arthritis
Flavonoids
biology
Traditional medicine
business.industry
Plant Extracts
Anti-Inflammatory Agents, Non-Steroidal
General Medicine
U937 Cells
Anti- inflammatory
biology.organism_classification
medicine.disease
0104 chemical sciences
Gout
Rats
010404 medicinal & biomolecular chemistry
030104 developmental biology
Fluorescence Molecular Tomography (FMT)
Complementary and alternative medicine
chemistry
Rheumatoid arthritis
medicine.symptom
Inflammation Mediators
business
Research Article
Subjects
Details
- ISSN :
- 14726882
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC complementary and alternative medicine
- Accession number :
- edsair.doi.dedup.....589d9825f495270b0d21a4782f2a5939