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The Effect of Myostatin (GDF-8) on Proliferation and Tenocyte Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells
- Source :
- The Journal of Hand Surgery (Asian-Pacific Volume). 22:200-207
- Publication Year :
- 2017
- Publisher :
- World Scientific Pub Co Pte Lt, 2017.
-
Abstract
- Background: The future in flexor tendon surgery involves tissue engineering approaches directed toward increasing early repair strength to accelerate tendon healing and to allow for earlier onset of rehabilitation. Previous work has shown that pluripotential mesenchymal stem cells may be successfully delivered to a tendon repair site using a suture carrier. The current work describes the use of Myostatin (GDF-8) to help guide these delivered pluripotential stem cells to differentiate down a tenocyte lineage to potentially maximize the reparative effects of these cells at the tendon repair site.Methods: Primary rat bone marrow mesenchymal stem cells isolated from the long bones of male Sprague-Dawley rats were treated with 500 ng/ml myostatin for 24 h, 48 h, and 72 h. Collagen 1 A, scleraxis (Scx), and tenomodulin (Tnmd) expression, indicative of tenogenesis, was analyzed using real time PCR and immunohistochemistry staining. A migration assay was performed to assess the functional activity of BMSCs after they were treated with myostatin.Results: Compared to the control cells (without treatment), the cells treated with 500 ng/ml myostatin for 72 h exhibited higher expression of Col 1A, Scx, and Tnmd. The mRNA expression of Col1A, Scx, Tnmd increased 15.3, 13 and 7 times respectively. Immunohistochemistry staining showed Scx and Tnmd were expressed in the cellular cytoplasm. In response to myostatin, the cells also showed a tendency to proliferate and migrate more than the control cells.Conclusions: Myostatin (GDF-8) has the ability to increase rat bone marrow mesenchymal stem cell growth and differentiation toward a tenocyte lineage. This information could be useful for future studies regarding tendon repair.
- Subjects :
- Male
0301 basic medicine
Cell Culture Techniques
Myostatin
Collagen Type I
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Tissue engineering
Basic Helix-Loop-Helix Transcription Factors
medicine
Animals
Cell Proliferation
030222 orthopedics
biology
business.industry
Scleraxis
Mesenchymal stem cell
Membrane Proteins
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
musculoskeletal system
Rats
Tendon
Tenomodulin
Cell biology
Tenocytes
Collagen, type I, alpha 1
030104 developmental biology
medicine.anatomical_structure
Immunology
biology.protein
Stem cell
business
Subjects
Details
- ISSN :
- 24248363 and 24248355
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- The Journal of Hand Surgery (Asian-Pacific Volume)
- Accession number :
- edsair.doi.dedup.....58a5011d95f277f05715c2ad2e369169