Additional file 1 of Biodegradable nanoplatform upregulates tumor microenvironment acidity for enhanced cancer therapy via synergistic induction of apoptosis, ferroptosis, and anti-angiogenesis

Additional file 1: Figure S1. A) The hydrodynamic size of FePt, MOFePt, pLMOFePt-TGO. B) Hydrodynamic size change of pLMOFePt-TGO pLMOFePt-TGO in a different medium. Figure S2.The particle size and polymer dispersity index (PDI) change of pLMOFePt-TGO in (A) distilled water, (B) PBS, and (C) DMEM with standing time. Figure S3. A-F) XPS high-resolution spectra of Fe2p, Pt4f, Si2p, S2p C1s, and full spectra in pLMOFePt-TGO. Figure S4. Zeta potential of FePt, MOFePt,pLMOFePt-TGO. Figure S5. A) Fourier transform infrared spectroscopy of pLMOFePt-TGO. B) Thermogravimetric curve of TAM, MOFePt, and MOFeP-T. C) The UV-vis standard curve of GOx. D) The loading rate of FePt and GOx calculated by ICP-MS analysis and UV-vis standard curve of GOx in E). Figure S6. A) pH value change of different concentration glucose solution treated with pLMOFePt-TGO. B) Cumulative release profile of FePt from pLMOFePt in various pH solutions. The internalization of pLMOFePt in MCF-7 cells: C) low magnification of Bio-TEM observation and D) high magnification of Bio-TEM observation. Figure S7. A) Pharmacokinetic curves of FePt and pLMOFePt analyzed by ICP-MS. B) The standard curve of intracellular acidity obtained by fluorescent probe BCECF-AM. C) The fluorescence spectrum of MCF-7 cells stained with BCECF-AM to assess cellular acidity. Figure S8. The viability of MCF-7 cells treated with pLMOFePt-TGO at the absence and presence of (A) DFOM, (B) Fer-1, (C) NAC, and (D) Z-VAD-FMK. Figure S9. The images of 4T1 cells stained with JC-1 A) and BODIPY B), respectively after treatment with FePt and pLMOFePt-TGO. Figure S10. (A) The tube formation of C166 cells treated with different concentrations of pLMOFePt-TGO; (B) corresponding number of tube formation. Figure S11. H&E staining images of the vital organs in different treatment groups. Figure S12. Ki67 staining images of MCF-7 tumor-bearing mice treated with different samples.