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CIDE domains form functionally important higher-order assemblies for DNA fragmentation

Authors :
Jae-Hee Jeong
Chang Min Kim
Yeon-Gil Kim
Hao Wu
Se Hoon Hong
Yong-Keun Jung
Hyun Ho Park
Qi Qiao
Jae Young Choi
Source :
Proceedings of the National Academy of Sciences. 114:7361-7366
Publication Year :
2017
Publisher :
Proceedings of the National Academy of Sciences, 2017.

Abstract

Significance Cell death-inducing DFF45-like effector (CIDE) domains, initially identified in apoptotic nucleases, form a highly conserved family with diverse functions ranging from cell death to lipid homeostasis and synaptic regulation. Through structural determination of two CIDE family proteins, Drep2 and Drep4, we found that CIDE domains can form helical oligomers. Our results reveal that such higher-order structures not only are conserved in the CIDE family, but also are critically important for both DNA fragmentation and lipid droplet fusion. Therefore, our findings identify the CIDE domain as a scaffolding component for higher-order structure assembly. Our results expand the importance of higher-order structures from the established field of immune signaling to broader biological functions.

Details

ISSN :
10916490 and 00278424
Volume :
114
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences
Accession number :
edsair.doi.dedup.....58d09572c905816da26897af8b8d7809
Full Text :
https://doi.org/10.1073/pnas.1705949114