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Prevalence of Yersinia Species in the Ileum of Crohn's Disease Patients and Controls
- Source :
- Frontiers in Cellular and Infection Microbiology, Frontiers in Cellular and Infection Microbiology, Frontiers Media, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩, Frontiers in Cellular and Infection Microbiology, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩, Frontiers in Cellular and Infection Microbiology (8), . (2018), Frontiers in Cellular and Infection Microbiology, Vol 8 (2018), Frontiers in Cellular and Infection Microbiology, Frontiers, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; Yersinia are common contaminants of food products, but their prevalence in the human gut is poorly documented. Yersinia have been implicated in Crohn's Disease (CD, an inflammatory bowel disease) however their role in CD is controversial. We performed highly sensitive PCR assays of specific sequences for the gyrB gene of Y. aldovae, Y. bercovieri, Y. enterocolitica, Y. intermedia, Y. mollaretii and the inv gene of Y. pseudotuberculosis. We analyzed a total of 470 ileal samples taken from 338 participants (262 CD patients and 76 controls) belonging to three independent cohorts. All patients and controls were phenotyped and genotyped for the main CD susceptibility variants: NOD2, ATG16L1, and IRGM. Yersinia were found in 7.7% of ileal samples (respectively 7.9 and 7.6% in controls and CD patients) corresponding to 10% of participants (respectively 11.8 and 9.5% in controls and CD patients). Y. enterocolitica, Y. pseudotuberculosis and Y. intermedia were the most frequently identified species. The bacteria were more frequent in resected specimens, lymph nodes and Peyer's patches. Yersinia were no more likely to be detected in CD tissues than tissues from inflammatory and non-inflammatory controls. CD patients treated with immunosuppressants were less likely to be Yersinia carriers. In conclusion, this work shows that Yersinia species are frequently found at low levels in the human ileum in health and disease. The role of Yersinia species in this ecosystem should now be explored.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
système immunitaire
Immunology
lcsh:QR1-502
Ileum
Biology
Gut flora
Yersinia
Microbiology
Inflammatory bowel disease
lcsh:Microbiology
yersinia
03 medical and health sciences
0302 clinical medicine
NOD2
Immunologie
medicine
mucosal immune system
ATG16L1
innate immunity
Crohn's disease
gut microbiota
ileal mucosa
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
maladie de crohn
medicine.disease
biology.organism_classification
molecular test
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
ileon
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
microflore digestive
IRGM
030211 gastroenterology & hepatology
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular and Infection Microbiology, Frontiers in Cellular and Infection Microbiology, Frontiers Media, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩, Frontiers in Cellular and Infection Microbiology, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩, Frontiers in Cellular and Infection Microbiology (8), . (2018), Frontiers in Cellular and Infection Microbiology, Vol 8 (2018), Frontiers in Cellular and Infection Microbiology, Frontiers, 2018, 8, ⟨10.3389/fcimb.2018.00336⟩
- Accession number :
- edsair.doi.dedup.....58db1e2b2257e1f19b0be273862eaba4