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Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles
- Source :
- Journal of Hazardous Materials. 399:122809
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Zinc oxide nanoparticles (ZnO-NPs) have been extensively applied in our daily life. Humans are at high risk of being exposed to ZnO-NPs, which induce potentially adverse health effects. Although a growing number of studies have investigated the toxic effects of ZnO-NPs, the available data concerning ZnO-NP interactions with the blood-milk barrier (BMB) remain highly limited. Herein, we systematically investigated the damage to BMB integrity induced by ZnO-NPs and the mechanisms involved. ZnO-NPs that were intravenously injected into lactating dams accumulated in the mammary gland and entered into the breast milk, inducing disruption to BMB integrity and changes in the tight junction (TJ) and adherens junction (AJ) components. Furthermore, using an in vitro BMB model composed of EpH4-Ev cells, we verified that ZnO-NP-triggered ROS generation and the activation of MKK4 and JNK are the main mechanism of cell-cell junction damage. More interestingly, JNK activation played different roles in inducing changes in the TJ and AJ complex, and these effects did not need to activate the downstream c-Jun. These data provide more information for understanding ZnO-NP interactions with the BMB and raise concern for the daily use and the intravenous use of ZnO-NPs by lactating mothers.
- Subjects :
- Environmental Engineering
Cell Survival
Health, Toxicology and Mutagenesis
Mammary gland
0211 other engineering and technologies
chemistry.chemical_element
02 engineering and technology
Zinc
010501 environmental sciences
01 natural sciences
Adherens junction
Intravenous use
Adverse health effect
medicine
Animals
Humans
Lactation
Environmental Chemistry
Waste Management and Disposal
0105 earth and related environmental sciences
021110 strategic, defence & security studies
Tight junction
Chemistry
Pollution
In vitro
Cell biology
Milk
medicine.anatomical_structure
Nanoparticles
Female
Zinc Oxide
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 03043894
- Volume :
- 399
- Database :
- OpenAIRE
- Journal :
- Journal of Hazardous Materials
- Accession number :
- edsair.doi.dedup.....58f9b9ac5117c81371ba310048ce001b