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The Arabidopsis KH-domain protein FLOWERING LOCUS Y delays flowering by upregulating FLOWERING LOCUS C family members
- Source :
- Plant Cell Reports. 39:1705-1717
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- We identified FLY as a previously uncharacterized RNA-binding-family protein that controls flowering time by positively regulating the expression of FLC clade members. The ability of flowering plants to adjust the timing of the floral transition based on endogenous and environmental signals contributes to their adaptive success. In Arabidopsis thaliana, the MADS-domain protein FLOWERING LOCUS C (FLC) and the FLC clade members FLOWERING LOCUS M/MADS AFFECTING FLOWERING1 (FLM/MAF1), MAF2, MAF3, MAF4, and MAF5 form nuclear complexes that repress flowering under noninductive conditions. However, how FLM/MAF genes are regulated requires further study. Using a genetic strategy, we showed that the previously uncharacterized K-homology (KH) domain protein FLOWERING LOCUS Y (FLY) modulates flowering time. The fly-1 knockout mutant and FLY artificial microRNA knockdown line flowered earlier than the wild type under long- and short-day conditions. The knockout fly-1 allele, a SALK T-DNA insertion mutant, contains an ~ 110-kb genomic deletion induced by T-DNA integration. FLC clade members were downregulated in the fly-1 mutants and FLY artificial microRNA knockdown line, whereas the level of the FLC antisense transcript COOLAIR was similar to that of the wild type. Our results identify FLY as a regulator that affects flowering time through upregulation of FLC clade members.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Mutant
Arabidopsis
MADS Domain Proteins
Locus (genetics)
Flowers
Plant Science
01 natural sciences
03 medical and health sciences
Gene Expression Regulation, Plant
hemic and lymphatic diseases
Flowering Locus C
Allele
Gene
Cell Nucleus
Genetics
biology
Arabidopsis Proteins
fungi
Wild type
food and beverages
General Medicine
Plants, Genetically Modified
biology.organism_classification
KH domain
Circadian Rhythm
Up-Regulation
030104 developmental biology
Gene Knockdown Techniques
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1432203X and 07217714
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Plant Cell Reports
- Accession number :
- edsair.doi.dedup.....58fc37e20d3dfe4f748c5b47b4f05bad