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T cells and the thymus in developing zebrafish
- Source :
- Developmental and comparative immunology. 28(7-8)
- Publication Year :
- 2003
-
Abstract
- The expression of genes encoding T cell receptor (TCR) alpha was used to follow the development of the thymus and to analyze the distribution of T cells in zebrafish. In the thymus, expression was first detected, by in situ hybridization, at four days post fertilization. In RNA extracted from whole fish, TCRalpha transcripts were also detected at four days and reached adult levels at three weeks. At six weeks, TCRalpha was expressed throughout the thymus, whereas rag1 expression was localized to the peripheral regions. Expression of TCRalpha outside the thymus was detected at nine days. In adult peripheral organs, the greatest expression was in the pronephros, mesonephros and intestine; expression in the spleen became greater as fish age. Three new, relatively highly expressed, TCR Valpha families were identified.
- Subjects :
- Embryo, Nonmammalian
T-Lymphocytes
Immunology
Molecular Sequence Data
Receptors, Antigen, T-Cell
Spleen
In situ hybridization
Thymus Gland
Biology
Recombination-activating gene
medicine
Animals
Amino Acid Sequence
Intestinal Mucosa
Zebrafish
In Situ Hybridization
Regulation of gene expression
Homeodomain Proteins
Mesonephros
T-cell receptor
Gene Expression Regulation, Developmental
biology.organism_classification
Molecular biology
Pronephros
Intestines
medicine.anatomical_structure
Developmental Biology
Subjects
Details
- ISSN :
- 0145305X
- Volume :
- 28
- Issue :
- 7-8
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....5908bf1347b0e0e06c173aaca8d2d9fd