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Protection against experimental small intestinal ischaemia–reperfusion injury with oxygenated perfluorochemical
- Source :
- British Journal of Surgery. 90:1015-1020
- Publication Year :
- 2003
- Publisher :
- Oxford University Press (OUP), 2003.
-
Abstract
- Background Intestinal ischaemia–reperfusion (IR) injury frequently occurs in abdominal surgery. Perfluorochemical (PFC) can be used to oxygenate intestinal organs directly and allows adenosine 5′-triphosphate (ATP) production within the submerged organs during ischaemia. This study was designed to evaluate the protective effect of PFC in IR injury, focusing on cytokine production in rat small intestine. Methods The superior mesenteric artery was occluded in rats for 60 min and the small bowel placed in an intestinal bag containing either normal saline (group 1), oxygenated saline (group 2) or oxygenated PFC (group 3). The arterial clip was subsequently removed, allowing reperfusion. The number of rats that survived for 7 days, tissue ATP levels, biochemical variables, tissue lipid peroxidation (LPO), bacterial cultures and histological changes were examined after reperfusion. Results The use of oxygenated PFC in group 3 improved survival compared with the other groups. Serum creatine phosphokinase and lactate dehydrogenase levels in groups 1 and 2 reflected small intestinal damage, and plasma levels of tumour necrosis factor α and interleukin 6 were raised. In contrast, oxygenated PFC decreased these levels, and reduced LPO, bacterial translocation and augmented apoptosis of the small intestine after reperfusion. Conclusion An intestinal bag containing oxygenated PFC showed protective effects during bowel ischaemia.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Necrosis
medicine.medical_treatment
Ischemia
chemistry.chemical_compound
Adenosine Triphosphate
Reperfusion therapy
medicine.artery
Lactate dehydrogenase
Internal medicine
Intestine, Small
medicine
Animals
Superior mesenteric artery
Rats, Wistar
Creatine Kinase
Saline
Fluorocarbons
Tumor Necrosis Factor-alpha
business.industry
medicine.disease
Survival Analysis
Small intestine
Rats
Endocrinology
medicine.anatomical_structure
chemistry
Reperfusion Injury
Surgery
Lipid Peroxidation
medicine.symptom
business
Reperfusion injury
Subjects
Details
- ISSN :
- 13652168 and 00071323
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- British Journal of Surgery
- Accession number :
- edsair.doi.dedup.....59143488d7a7b2c22781d1cb6393cfb6
- Full Text :
- https://doi.org/10.1002/bjs.4138