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PVT1‐derived miR‐1207‐5p promotes breast cancer cell growth by targeting STAT6
- Source :
- Cancer Science
- Publication Year :
- 2017
- Publisher :
- John Wiley and Sons Inc., 2017.
-
Abstract
- Accumulating evidence indicates that ectopic expression of non-coding RNAs are responsible for breast cancer progression. Increased non-coding RNA PVT1, the host gene of microRNA-1207-5p (miR-1207-5p), has been associated with breast cancer proliferation. However, how PVT1 functions in breast cancer is still not clear. In this study, we show a PVT1-derived microRNA, miR-1207-5p, that promotes the proliferation of breast cancer cells by directly regulating STAT6. We first confirm the positive correlated expression pattern between PVT1 and miR-1207-5p by observing consistent induced expression by estrogen, and overexpression in breast cancer cell lines and breast cancer patient specimens. Moreover, silence of PVT1 also decreased miR-1207-5p expression. Furthermore, increased miR-1207-5p expression promoted, while decreased miR-1207-5p expression suppressed, cell proliferation, colony formation, and cell cycle progression in breast cancer cell lines. Mechanistically, a novel target of miR-1207-5p, STAT6, was identified by a luciferase reporter assay. Overexpression of miR-1207-5p decreased the levels of STAT6, which activated CDKN1A and CDKN1B to regulate the cell cycle. We also confirmed the reverse correlation of miR-1207-5p and STAT6 expression levels in breast cancer samples. Therefore, our findings reveal that PVT1-derived miR-1207-5p promotes the proliferation of breast cancer cells by targeting STAT6, which in turn controls CDKN1A and CDKN1B expression. These findings suggest miR-1207-5p might be a potential target for breast cancer therapy.
- Subjects :
- 0301 basic medicine
Cancer Research
medicine.drug_class
Carcinogenesis
Breast Neoplasms
Biology
Cell Line
03 medical and health sciences
0302 clinical medicine
Breast cancer
Cell Movement
Cell Line, Tumor
microRNA
medicine
Humans
Breast
PVT1
STAT6
Cell growth
Cell Cycle
miR‐1207‐5p
General Medicine
Original Articles
Cell cycle
Middle Aged
medicine.disease
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
cell proliferation
Cell Transformation, Neoplastic
HEK293 Cells
Oncology
Estrogen
030220 oncology & carcinogenesis
Cancer research
Ectopic expression
Original Article
RNA, Long Noncoding
CDKN1B
STAT6 Transcription Factor
Subjects
Details
- Language :
- English
- ISSN :
- 13497006 and 13479032
- Volume :
- 108
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....594522c01fb111c5e2417fc9e566a00e