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MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE, Vol 16, Iss 11, p e0260071 (2021)
- Publication Year :
- 2021
-
Abstract
- Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3’UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.
- Subjects :
- Gene Expression
Apoptosis
Cell Communication
Retinal Pigment Epithelium
Biochemistry
Medical Conditions
Medicine and Health Sciences
3' Untranslated Regions
Immune Response
Cell Analysis
Gene knockdown
Multidisciplinary
medicine.diagnostic_test
Cell Death
Chemistry
Organic Compounds
Monosaccharides
Nucleic acids
Real-time polymerase chain reaction
Bioassays and Physiological Analysis
Cell Processes
Physical Sciences
Medicine
Hyperexpression Techniques
Research Article
Glia Maturation Factor
Cell Viability Testing
Cell Survival
Science
Immunology
Carbohydrates
Glia maturation factor
Transfection
Research and Analysis Methods
Flow cytometry
Cell Line
Signs and Symptoms
Phagocytosis
microRNA
medicine
Gene Expression and Vector Techniques
Genetics
Humans
Secretion
Viability assay
Molecular Biology Techniques
Non-coding RNA
Molecular Biology
Inflammation
Molecular Biology Assays and Analysis Techniques
Natural antisense transcripts
Diabetic Retinopathy
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Cell Biology
Molecular biology
Gene regulation
MicroRNAs
Glucose
Gene Expression Regulation
Hyperglycemia
Metabolic Disorders
RNA
Clinical Medicine
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....5966d9c9ee65036cd96c27763e29e4bb