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Reconstitution of EPA and DHA biosynthesis in Arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants
- Source :
- Metabolic Engineering
- Publication Year :
- 2013
- Publisher :
- Elsevier, 2013.
-
Abstract
- An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid metabolism. Successive iterations were informed by lipidomic analysis of neutral, polar and acyl-CoA pools. This approach allowed for a four-fold improvement on levels previously reported for the accumulation of eicosapentaenoic acid in Arabidopsis seeds and also facilitated the successful engineering of the high value polyunsaturated fatty acid docosahexaenoic acid to 10-fold higher levels. Our studies identify the minimal gene set required to direct the efficient synthesis of these fatty acids in transgenic seed oil.<br />Highlights ► Successful iteration of EPA and DHA accumulation in transgenic plants. ► Systematic identification of optimal genes for accumulation of DHA. ► Demonstration of the utility of lipidomic-driven plant metabolic engineering.
- Subjects :
- PC, Phosphatidylcholine
Docosahexaenoic Acids
Desaturase
Transgene
Arabidopsis
LC-PUFA, Long chain polyunsaturated fatty acid
PS, Phosphatidylserine
Bioengineering
Biology
Applied Microbiology and Biotechnology
Article
Metabolic engineering
chemistry.chemical_compound
DAG, Diacylglycerol
Fatty Acids, Omega-3
Arabidopsis thaliana
PE, Phosphatidylethanolamine
Nutritional enhancement
DPA, Docosapentaenoic acid
TAG, Triacylglycerol
chemistry.chemical_classification
LA, Linoleic acid
Fatty acid metabolism
PI, Phosphatidylinositol
Transgenic plants
Plants, Genetically Modified
biology.organism_classification
DHA, Docosahexaenoic acid
Eicosapentaenoic acid
EPA, Eicosapentaenoic acid
Elongase
SDA, Stearidonic acid
Genetic Enhancement
Eicosapentaenoic Acid
Metabolic Engineering
ARA, Arachidonic acid
chemistry
Biochemistry
Docosahexaenoic acid
ALA, α-Linolenic acid
GLA, γ-Linolenic acid
Signal Transduction
Omega-3 long chain polyunsaturated fatty acids
Biotechnology
Polyunsaturated fatty acid
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Metabolic Engineering
- Accession number :
- edsair.doi.dedup.....59d48e564e2fe2741c275c8a0056932c