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Histological and ultrastructural changes of the colon in dextran sodium sulfate‑induced mouse colitis
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2020
- Publisher :
- Spandidos Publications, 2020.
-
Abstract
- Ulcerative colitis (UC) is a complex disease that results from a dysregulated immune response in the gastrointestinal tract. A mouse model orally administered with dextran sodium sulfate (DSS) is the most widely used experimental animal model of UC. However, the ultrastructure of the colon in mouse colitis is poorly understood. In the present study, colonic specimens from DSS-induced UC mice underwent hematoxylin and eosin staining, Masson's trichrome staining and Verhoeff's elastic staining. In addition, the ultrastructure of samples was examined by transmission electron microscopy. UC was successfully induced by 7 consecutive days of DSS oral administration. The goblet cell architecture of the UC tissue was damaged in the mucosa. Additionally, a significant number of inflammatory cells infiltrated into the stroma and the structure of the intestinal gland was destroyed. The tissue in the submucosa showed significant edema. Hyperplasia was also identified in the submucosa, promoting a disorganized microstructure within the colon wall. Numerous collagen fibers in the muscular layer were disrupted, and the fiber bundles were thinner compared with those in the normal control group. Furthermore, in the DSS-induced UC group, the smooth muscle cell showed edema, the cell membrane structure was unclear and the shape of the nucleus was irregular. In conclusion, the present study revealed important histological and ultrastructural changes in the colon of DSS-induced UC mice. These features may contribute to improved understanding of the pathogenesis and mechanism of UC.
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
H&E stain
histology
Masson's trichrome stain
Muscular layer
03 medical and health sciences
0302 clinical medicine
Immunology and Microbiology (miscellaneous)
Submucosa
medicine
Colitis
Goblet cell
colon
Chemistry
Articles
General Medicine
dextran sodium sulfate-induced ulcerative colitis
medicine.disease
ultrastructure
Ulcerative colitis
digestive system diseases
Staining
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Subjects
Details
- ISSN :
- 17921015 and 17920981
- Database :
- OpenAIRE
- Journal :
- Experimental and Therapeutic Medicine
- Accession number :
- edsair.doi.dedup.....5a28a1fba98f7c1d5b868dc2bd164d65