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Evidence that the cytolethal distending toxin locus was once part of a genomic island in the periodontal pathogen Aggregatibacter (Actinobacillus) actinomycetemcomitans strain Y4
- Source :
- Journal of Medical Microbiology. 56:1519-1527
- Publication Year :
- 2007
- Publisher :
- Microbiology Society, 2007.
-
Abstract
- The authors have previously shown that the periodontal pathogen Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans Y4 contains an operon for a genotoxin known as the cytolethal distending toxin (Cdt). The cdt locus in strain Y4 is flanked by remnants of heterologous plasmid and integrase sequences. In this study, the DNA sequence immediately downstream from the cdt locus on the Y4 chromosome was examined. The extended sequence contained a region that had all the characteristics of a typical bacterial pathogenicity or genomic island. The genomic island (GIY4-1) was approximately 22 kb long, was flanked by a bacteriophage attachment (att) sequence and contained a full-length integrase/resolvase gene (xerD). A total of 22 complete and partial ORFs represented putative DNA replication/DNA binding/conjugation proteins as well as hypothetical proteins. GIY4-1 was most closely related to putative genomic islands in Haemophilus ducreyi 35000HP and Haemophilus influenzae 86-028NP and to a chromosomal region in Haemophilus somnus 129PT. GIY4-1 was not present in HK1651, which was used as the prototype strain for genomic sequencing of A. actinomycetemcomitans. Several sequences in GIY4-1 were homologous to ORFs found on the A. actinomycetemcomitans plasmid pVT745. None of the identified ORFs in GIY4-1 appeared to encode potential virulence genes. However, several unique observations supported the possibility that the cdt locus of A. actinomycetemcomitans Y4 was originally contained within the genomic island.
- Subjects :
- DNA Replication
DNA, Bacterial
Microbiology (medical)
Genomic Islands
Cytolethal distending toxin
Bacterial Toxins
Molecular Sequence Data
Sequence Homology
Locus (genetics)
Aggregatibacter actinomycetemcomitans
Microbiology
Article
Haemophilus ducreyi
Open Reading Frames
Plasmid
Bacterial Proteins
Genomic island
Gene Order
Haemophilus somnus
ORFS
Gene
Genetics
Integrases
biology
Sequence Analysis, DNA
General Medicine
biology.organism_classification
Haemophilus influenzae
DNA-Binding Proteins
Conjugation, Genetic
Attachment Sites, Microbiological
Chromosomal region
Plasmids
Subjects
Details
- ISSN :
- 14735644 and 00222615
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Microbiology
- Accession number :
- edsair.doi.dedup.....5a331043713932c511244e3e1bfc0a3b
- Full Text :
- https://doi.org/10.1099/jmm.0.47273-0