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Synergy of pancratistatin and tamoxifen on breast cancer cells in inducing apoptosis by targeting mitochondria

Authors :
Carly Griffin
Siyaram Pandey
James McNulty
Sridhar S. Tirumalai
Peter Siedlakowski
Amanda McLachlan-Burgess
Source :
Cancer Biology & Therapy. 7:376-384
Publication Year :
2008
Publisher :
Informa UK Limited, 2008.

Abstract

Pancratistatin (PST), a natural compound obtained from the Hawaiian spider lily, is known to be specific and selective in inducing apoptosis in multiple cancer cell lines while sparing non-cancerous cells and cell lines. Here we report the ability of PST to induce apoptosis specifically in human breast cancer cell lines MCF-7 and Hs-578-T compared to their non cancerous counterparts. In cancer cells PST caused increased levels of reactive oxygen species (ROS), decreased ATP and mitochondrial membrane permeabilization indicating the activation of the mitochondrial pathway of apoptosis. In combination with the anti-estrogen Tamoxifen, PST had a synergic effect. Both compounds caused increased production of ROS when applied to isolated mitochondria from these cancer cell lines supporting the observation that Tamoxifen might work through mechanisms distinct from the canonical estrogen receptor antagonism.

Details

ISSN :
15558576 and 15384047
Volume :
7
Database :
OpenAIRE
Journal :
Cancer Biology & Therapy
Accession number :
edsair.doi.dedup.....5a334719617d851356a013e9853becee
Full Text :
https://doi.org/10.4161/cbt.7.3.5364