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Deductive analysis of a protein-synthesis mutant of Escherichia coli
- Source :
- Biochemical Genetics. 8:205-230
- Publication Year :
- 1973
- Publisher :
- Springer Science and Business Media LLC, 1973.
-
Abstract
- A mutant of E. coli, Ts68b, selected as unable to grow at 43 C, is unable to synthesize proteins at 43 C, though it can carry out this function at 30 C. This defect is shown to be in the protein-synthetic rather than the RNA-synthetic machinery by an analysis of the response of the strain to infection with the RNA bacteriophage f2. An analysis of the capacity for RNA synthesis and the polyribosome content of these cells at 44 C indicates that the defect resides in the elongation step of protein synthesis. No defects could be detected in vitro. The results are discussed in light of similar data on other mutants and in relation to the general approach of analyzing complex mutants selected with ill-defined phenotypes.
- Subjects :
- Genetics, Microbial
Peptide Biosynthesis
Time Factors
Mutant
Cell Fractionation
Tritium
medicine.disease_cause
Coliphages
Biochemistry
Bacterial Proteins
Polysome
Escherichia coli
Genetics
medicine
Protein biosynthesis
Amino Acids
Uridine
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Cell-Free System
biology
Strain (chemistry)
Temperature
Chromosome Mapping
RNA
General Medicine
biology.organism_classification
Molecular biology
In vitro
Kinetics
RNA, Bacterial
Phenotype
Polyribosomes
Mutation
Bacteriophage f2
Subjects
Details
- ISSN :
- 15734927 and 00062928
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Biochemical Genetics
- Accession number :
- edsair.doi.dedup.....5a3f35c792ef2bd35fffdbde6f1fc843