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Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes
- Source :
- Methods in Molecular Biology ISBN: 9781493985692
- Publication Year :
- 2018
- Publisher :
- Springer New York, 2018.
-
Abstract
- There is a diverse population of mononuclear phagocytes (MPs) in the lungs, comprised of macrophages, dendritic cells (DCs), and monocytes. The existence of these various cell types suggests that there is a clear division of labor and delicate balance between the MPs under steady-state and inflammatory conditions. Here we describe how to identify pulmonary MPs using flow cytometry and how to isolate them via cell sorting. In steady-state conditions, murine lungs contain a uniform population of alveolar macrophages (AMs), three distinct interstitial macrophage (IM) populations, three DC subtypes, and a small number of tissue-trafficking monocytes. During an inflammatory response, the monocyte population is more abundant and complex since it acquires either macrophage-like or DC-like features. All in all, studying how these cell types interact with each other, structural cells, and other leukocytes within the environment will be important to understanding their role in maintaining homeostasis and during the development of disease.
- Subjects :
- 0301 basic medicine
Cell type
Population
Cell Separation
Biology
Monocytes
Article
Immunophenotyping
Flow cytometry
Mice
03 medical and health sciences
Macrophages, Alveolar
medicine
Animals
Macrophage
education
Lung
Phagocytes
education.field_of_study
medicine.diagnostic_test
Monocyte
Dendritic cell
Mononuclear phagocyte system
Cell sorting
Flow Cytometry
Cell biology
Phenotype
030104 developmental biology
medicine.anatomical_structure
Leukocytes, Mononuclear
Lymph Nodes
Biomarkers
Subjects
Details
- ISBN :
- 978-1-4939-8569-2
- ISBNs :
- 9781493985692
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493985692
- Accession number :
- edsair.doi.dedup.....5a4b00276b5306cefed1c3ee6434e6a6